Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 100G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 100G
Hampton Research StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
Chem-Impex International, Inc. 3-N-Morpholinopropanesulfonic acid | 1132-61-2 | MFCD00006183 | 250G
3-N-Morpholinopropanesulfonic acid, 1132-61-2, MFCD00006183, 250G
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659771.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 1 Kg
PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a highly effective buffer used in a wide range of biochemical and molecular biology applications. This 1kg USP-grade bottle provides enough reagent for preparing larger quantities of pH-stable solutions. PIPES is particularly useful in situations where other commonly used buffers, such as phosphate, cannot maintain their pH under high salt conditions or at extreme temperatures. This buffer is essential for many laboratory protocols, including protein purification, enzyme assays, and cell culture studies. The large volume is perfect for high-throughput research and provides significant cost savings for laboratories that require large amounts of buffer solution.
Sigma Aldrich Fine Chemicals Biosciences MES hemisodium salt 98 tit100G
SODIUM MES hemisodium salt 98 tit100G
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 25 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is ideal for laboratory applications requiring precise pH regulation. ADA is commonly used in chromatography, protein studies, and enzyme assays, providing a stable pH environment during various biochemical experiments. Its ability to maintain a controlled pH is essential for accurate results in protein purification, cell culture, and molecular biology studies. The 25g size is perfect for smaller-scale experiments or routine laboratory use where reliable buffering capacity is necessary for optimal results.