Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ Sterile Saline, 0.9%, 1L
Introducing CellPro™ Sterile Saline, meticulously crafted by Alkali Scientific™ to meet the demanding standards of molecular biology applications. This Molecular Biology Grade saline solution ensures exceptional purity and sterility, making it ideal for various laboratory procedures, including cell culture, molecular assays, and biochemical experiments. With its precisely balanced formulation, CellPro™ Sterile Saline provides the essential ions and osmotic balance required for cell viability and physiological studies. Each batch undergoes rigorous quality control to guarantee consistency and reliability, empowering researchers with confidence in their results. Trust CellPro™ Sterile Saline to support your molecular biology research with unparalleled quality and performance.
Alkali Scientific CellPro™ Sterile Saline, 0.9%, 1L, 10/cs
Introducing CellPro™ Sterile Saline, meticulously crafted by Alkali Scientific™ to meet the demanding standards of molecular biology applications. This Molecular Biology Grade saline solution ensures exceptional purity and sterility, making it ideal for various laboratory procedures, including cell culture, molecular assays, and biochemical experiments. With its precisely balanced formulation, CellPro™ Sterile Saline provides the essential ions and osmotic balance required for cell viability and physiological studies. Each batch undergoes rigorous quality control to guarantee consistency and reliability, empowering researchers with confidence in their results. Trust CellPro™ Sterile Saline to support your molecular biology research with unparalleled quality and performance.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, with Tween 20, pH 8.0 powder
Tris Buffered Saline, with Tween 20, pH 8.0 powder
Med Vet International Irrigation Solution Saljet 0.9% Sodium Chloride, Preservative Free Not for Injection Unit Dose Vial 30 mL, 64938-009-01, 240/Case
0.9% sodium chloride weight by volume Each 30-mL vial has a twist-off top 40 vials per box
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 250g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 250g
LICOR Odyssey MES SDS Running Buffer Powder (pack of 5)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.
Bioworld ACES, 100 g
ACES, High Purity Grade N-[carbamoylmethyl]-2-Aminoethane-sulfonic acidpKa: (20°C): 6.8+0.1Appearance: White crystalsHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.1-7.5
Sigma Aldrich Fine Chemicals Biosciences Hepes|7365-45-9 | MFCD00006158 | 100g
Hepes | Purity: 99.5% (titration) | MW: 238.3 | 7365-45-9 | MFCD00006158 | 100g
Sigma Aldrich Fine Chemicals Biosciences MOPSO sodium salt 99 titra500G
MOPSO sodium salt 99 titra500G
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
Boston Bioproducts Inc PIPES Buffer (0.5 M, pH 7.0) - 100ml
The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.
Medchemexpress LLC 3-morpholino-2-hydroxypropanesulfonic acid | 68399-77-9 | MFCD00009629 | 99.7% | 225.26 g/mol | C7H15NO5S | 500 G
MOPSO is a zwitterionic biological buffer used to maintain stable near-neutral pH in biochemical and cell culture applications. It buffers effectively in the 6.2-7.6 pH range and is supplied as a high-purity powder with supporting analytical documentation for quality control.
- Useful pH range of 6.2-7.6.
- High purity suitable for research applications.
- Appropriate for biological and biochemical buffering needs.
- Supplied as a powder for convenient storage and handling.
- Analytical documentation available (COA, SDS, HNMR, RP-HPLC, MS).
Chem-Impex International, Inc. N-(2-Acetamido)-2-aminoethanesulfonic acid | 7365-82-4 | MFCD00008030 | 1KG
N-(2-Acetamido)-2-aminoethanesulfonic acid, 7365-82-4, MFCD00008030, 1KG
Sigma Aldrich Fine Chemicals Biosciences HEPES, 7365-45-9, MFCD00006158, 500g
Empirical Formula (Hill Notation) C8H18N2O4S, ≥99.5% (titration), Molecular Weight 238.30, Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-500.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.